The Analgesiometer Tail Flick test is a well-established method for evaluating the nociceptive (pain) response in laboratory animals, particularly rodents like mice and rats. This test measures the time it takes for an animal to withdraw its tail from a heat source, such as a radiant heat beam, after it is exposed to the thermal stimulus. This apparatus consists of a radiant heat source, usually an infrared lamp, positioned beneath a glass or metal surface. A restraint device is used to hold the animal in place, allowing only its tail to be exposed to the heat source. Animals should be acclimated to the testing environment before the experiment. The tail should be cleaned to remove any foreign substances, which could interfere with the test. The animal's tail is gently placed on the heat source (radiant lamp). The timer is stopped as soon as the animal withdraws its tail from the heat, indicating a reflexive pain response. The Analgesiometer Tail Flick test is a valuable tool for evaluating pain sensitivity in laboratory animals. While it has its limitations, it remains a widely used and important method for preclinical research in pain management and analgesic drug development. When conducted with care and consistency, this test contributes to our understanding of pain mechanisms and the development of novel pain-relief strategies.